Influence of GM1 on Nogo-A Expression in Cerebral Cortex for the Brain Trauma Rats

Objective: To observe the effects of the ganglioside (GM1) on Nogo-A expression in cerebral cortex for the rats which have brain trauma, to explore the possible mechanisms of GMl promoting the nerve repair of traumatic brain injury. Methods: SPF SD rats of 100 were randomly divided into four groups: no operation group (n = 10), sham operation group (n = 20), saline model group (n=35), GMI group (n=35). ELISA was used to detect Nogo-A expression in parietal cerebral cortex at different time. Results: By immunohistochemical method we observed in model group: the number Nogo-A positive cells increased at 24 hours in the model, and still slightly increased till 72 hours; in group GM1: the number Nogo-A of positive cells increased gradually in the early time, and became largest at24 h, then started to decrease, the application of GM1, after brain trauma, the increase in the number of Nogo-A positive cells was more delayed. Conclusions: the number of Nogo-A positive cells increased significantly after brain trauma, and inhibited the CNS regeneration after injury. After brain trauma, the ganglioside GM1 partially inhibited Nogo-A expression, and the mechanism might be through the stability of cell membrane, which played its neuroprotection roles in experimental traumatic brain injury in rats.